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OfflineHags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 6 months
Cambodian 400x Microscopy * 3
    #14207400 - 03/30/11 01:08 AM (12 years, 10 months ago)

I run a lab with some pretty cool equipment. Decided to scan a slide of my Cambodian spores at 40X, thought I'd share...

Anyone know if the spores will express autofluorescence? I have a fluorescent scanning microscope as well :thumbup:=)




Edited by Hags (04/05/11 06:11 PM)

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Offlineesquaredx
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14208740 - 03/30/11 10:35 AM (12 years, 10 months ago)

They look so neat under a microscope :thumbup:
Thanks for sharing.


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Offlineshroomie_glen
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Re: Cambodian 40x Microscopy [Re: esquaredx]
    #14215437 - 03/31/11 03:18 PM (12 years, 10 months ago)

those are ovoids for sure...
appresh the sharage man....


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Invisiblearis
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Re: Cambodian 40x Microscopy [Re: shroomie_glen]
    #14225112 - 04/02/11 08:55 AM (12 years, 10 months ago)

like little avocados!

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Invisiblemycoelf
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Re: Cambodian 40x Microscopy [Re: aris]
    #14225165 - 04/02/11 09:16 AM (12 years, 10 months ago)

:nothingtoadd:


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Offlinesolumvita
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Re: Cambodian 40x Microscopy [Re: mycoelf]
    #14225990 - 04/02/11 01:12 PM (12 years, 10 months ago)

Nice picture what camera are you using?  i doubt the spores will fluoresce but try and let us know.


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OfflineHags
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Registered: 08/22/10
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Re: Cambodian 40x Microscopy [Re: solumvita]
    #14231485 - 04/03/11 02:59 PM (12 years, 10 months ago)

Its not a camera, its a special Microscope used for Histopathology analysis... Its about 150,00$, don't think u'll want to buy one.

I'll def try the fluoro, its worth a shot.

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Offlinesolumvita
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14239125 - 04/04/11 10:32 PM (12 years, 10 months ago)

Quote:

Hags said:
Its not a camera, its a special Microscope used for Histopathology analysis... Its about 150,00$, don't think u'll want to buy one.





I feel so patronized - I just wanted to know what equipment you are using!  I'm quite sure I don't want to buy it.

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OfflineAlan RockefellerM
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14240432 - 04/05/11 06:34 AM (12 years, 10 months ago)

That is not 40x, it is probably 400x since a common eyepiece size is 10x.  Magnification = objective magnification * eyepiece magnification.

Can you add a scale?

What kind of camera did you use?  And what kind of scope?  Does it have a 100x oil immersion objective?

It is necessary to accurately measure the size of the spores to determine the species. 

Try the fluorescence scope too, you should at least be able to make the spores look colorful or cool or something.  Perhaps the fluorescence can help see the parts of the spore, or maybe certain fluorescent dyes can make those show up in cool colors.

Psilocybe cubensis spores are unusually large, for a Psilocybe.

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OfflineHags
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Re: Cambodian 40x Microscopy [Re: Alan Rockefeller]
    #14243356 - 04/05/11 06:10 PM (12 years, 10 months ago)

I apologize solumvita! I did not mean that to come across rude anyway whatsoever. Honestly, I am keeping the microscope name somewhat secret because there are only a very few of them ever sold and that can narrow down my laboratory, and consequently myself. sorry!! :crazy:

Also, yes AR is right, I meant 400x overall, I am used to just saying 40x around my lab... And yes, it has oil immersion, but its a bitch to say the least and I don't want to really mess with that =( But I promise I will have the fluoroscan done soon, been really busy lately and its something I can only get to when my actual lab work is done.

I am attaching some pictures of measurements of height and width... just a few. If i get some free time I'll run some algorithms and share some digital analysis results.  :peace:  :heart:








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OfflineHags
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Registered: 08/22/10
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14243539 - 04/05/11 06:39 PM (12 years, 10 months ago)

Just did a quick analysis, of these 19, average area per spore is 70.78um2


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OfflineAlan RockefellerM
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14246065 - 04/06/11 03:52 AM (12 years, 10 months ago)

Quote:

Hags said:
Just did a quick analysis, of these 19, average area per spore is 70.78um2






It is cool that you give thousandths of a micrometer.  This is clearly more accuracy than any light microscope has, but it makes it look good anyway.

We don't really use spore area in mycology, interesting analysis though.

We use mostly length, width and Q.  Q is calculated by dividing the length by the width.

To do it right, measure 30 spores and drop the two largest and two smallest spores.  Record the range of spore width, range of spore size, smallest spores and largest spores (after dropping the 2 largest and smallest).  The Q should be calculated for each spore and the range of Q should be given, and also the average Q.

The last picture in this observation is done pretty well:

http://mushroomobserver.org/64805



The numbers in parenthesis are the largest and smallest values observed.  N is the number of spores measured.  Me is the mean spore size and Qe is the mean Q value.

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OfflineHags
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Re: Cambodian 40x Microscopy [Re: Alan Rockefeller]
    #14249891 - 04/06/11 08:58 PM (12 years, 10 months ago)

Ya I had the area algorithm already handy. It will take a couple days to put together the Q, but damn it you've got me set on getting some real data out of this. As said, I do histopath data analysis for a living, and been published; with some legit results this is completely viable for a publication. will post soon, thanks AR

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OfflineWallpapereater
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14264137 - 04/09/11 07:53 PM (12 years, 10 months ago)

Really cool stuff man! :popcorn:


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OfflineTritium
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Re: Cambodian 40x Microscopy [Re: Wallpapereater]
    #14264188 - 04/09/11 08:02 PM (12 years, 10 months ago)

so as long as your work is finished you can use some of the lab equipment for non-work related things?

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Offlineic3.qu33n
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Re: Cambodian 40x Microscopy [Re: Tritium]
    #14316209 - 04/19/11 09:13 AM (12 years, 10 months ago)

thats sooo neat looking! i wanna be able to observe on that level someday.  Start small and build I guess.... I got a magnifying glass for now to view my rhizo growth...


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OfflineMr.E
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Re: Cambodian 40x Microscopy [Re: Alan Rockefeller]
    #14340883 - 04/23/11 10:00 PM (12 years, 10 months ago)

Very interesting Hags, thanks for posting and I hope you can continue your analysis. Your Cambodians seem to be on the small end of wiki claims for Psilocybe cubensis. "Microscopic features: Spores 11.5–17 x 8–11 µm". Not meant to disagree just found it interesting and relevant.

Wiki also claims "The diameter of human hair varies from 17 to 180 µm". I assume this would mean any visible Psilocybe 'spores' are actually clumps?

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Invisiblefngbronco
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Re: Cambodian 40x Microscopy [Re: Mr.E]
    #14347093 - 04/25/11 01:18 AM (12 years, 10 months ago)

One of the central spores in that first pic looks like it's growing out. That'd be awesome to put some agar between 2 slides and grow 2 spores out then transfer and get you an isolate!


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OfflineMolecularConcept
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Re: Cambodian 40x Microscopy [Re: fngbronco]
    #14347246 - 04/25/11 02:10 AM (12 years, 10 months ago)

This was a cool read!

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Offlinesingult
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Re: Cambodian 40x Microscopy [Re: MolecularConcept]
    #14347561 - 04/25/11 05:21 AM (12 years, 10 months ago)

Hags, much thanks for these! I've just started microscopy and what you're laying down is what I'm picking up, with gusto.
:thaaannks:

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