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Hags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 6 months
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Cambodian 400x Microscopy 3
#14207400 - 03/30/11 01:08 AM (12 years, 10 months ago) |
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I run a lab with some pretty cool equipment. Decided to scan a slide of my Cambodian spores at 40X, thought I'd share...
Anyone know if the spores will express autofluorescence? I have a fluorescent scanning microscope as well =)
Edited by Hags (04/05/11 06:11 PM)
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esquaredx



Registered: 10/24/09
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Re: Cambodian 40x Microscopy [Re: Hags]
#14208740 - 03/30/11 10:35 AM (12 years, 10 months ago) |
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They look so neat under a microscope Thanks for sharing.
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shroomie_glen
RedHotPussyLiquor



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Re: Cambodian 40x Microscopy [Re: esquaredx]
#14215437 - 03/31/11 03:18 PM (12 years, 10 months ago) |
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those are ovoids for sure... appresh the sharage man....
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No. No, man. Shit, no man. I believe you'd get your ass kicked sayin' somethin' like that man.
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aris
Feline Bovine


Registered: 07/20/10
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like little avocados!
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mycoelf
Agent Of Chaos



Registered: 06/26/09
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Re: Cambodian 40x Microscopy [Re: aris]
#14225165 - 04/02/11 09:16 AM (12 years, 10 months ago) |
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-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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solumvita
Q.B.E.


Registered: 02/12/08
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Re: Cambodian 40x Microscopy [Re: mycoelf]
#14225990 - 04/02/11 01:12 PM (12 years, 10 months ago) |
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Nice picture what camera are you using? i doubt the spores will fluoresce but try and let us know.
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
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Hags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 6 months
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Re: Cambodian 40x Microscopy [Re: solumvita]
#14231485 - 04/03/11 02:59 PM (12 years, 10 months ago) |
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Its not a camera, its a special Microscope used for Histopathology analysis... Its about 150,00$, don't think u'll want to buy one.
I'll def try the fluoro, its worth a shot.
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solumvita
Q.B.E.


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Re: Cambodian 40x Microscopy [Re: Hags]
#14239125 - 04/04/11 10:32 PM (12 years, 10 months ago) |
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Quote:
Hags said: Its not a camera, its a special Microscope used for Histopathology analysis... Its about 150,00$, don't think u'll want to buy one.
I feel so patronized - I just wanted to know what equipment you are using! I'm quite sure I don't want to buy it.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
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Re: Cambodian 40x Microscopy [Re: Hags]
#14240432 - 04/05/11 06:34 AM (12 years, 10 months ago) |
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That is not 40x, it is probably 400x since a common eyepiece size is 10x. Magnification = objective magnification * eyepiece magnification.
Can you add a scale?
What kind of camera did you use? And what kind of scope? Does it have a 100x oil immersion objective?
It is necessary to accurately measure the size of the spores to determine the species.
Try the fluorescence scope too, you should at least be able to make the spores look colorful or cool or something. Perhaps the fluorescence can help see the parts of the spore, or maybe certain fluorescent dyes can make those show up in cool colors.
Psilocybe cubensis spores are unusually large, for a Psilocybe.
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Hags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 6 months
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I apologize solumvita! I did not mean that to come across rude anyway whatsoever. Honestly, I am keeping the microscope name somewhat secret because there are only a very few of them ever sold and that can narrow down my laboratory, and consequently myself. sorry!! 
Also, yes AR is right, I meant 400x overall, I am used to just saying 40x around my lab... And yes, it has oil immersion, but its a bitch to say the least and I don't want to really mess with that =( But I promise I will have the fluoroscan done soon, been really busy lately and its something I can only get to when my actual lab work is done.
I am attaching some pictures of measurements of height and width... just a few. If i get some free time I'll run some algorithms and share some digital analysis results.



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Hags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 6 months
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Re: Cambodian 40x Microscopy [Re: Hags]
#14243539 - 04/05/11 06:39 PM (12 years, 10 months ago) |
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Just did a quick analysis, of these 19, average area per spore is 70.78um2
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,319
Last seen: 13 hours, 51 minutes
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Re: Cambodian 40x Microscopy [Re: Hags]
#14246065 - 04/06/11 03:52 AM (12 years, 10 months ago) |
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Quote:
Hags said: Just did a quick analysis, of these 19, average area per spore is 70.78um2
It is cool that you give thousandths of a micrometer. This is clearly more accuracy than any light microscope has, but it makes it look good anyway.
We don't really use spore area in mycology, interesting analysis though.
We use mostly length, width and Q. Q is calculated by dividing the length by the width.
To do it right, measure 30 spores and drop the two largest and two smallest spores. Record the range of spore width, range of spore size, smallest spores and largest spores (after dropping the 2 largest and smallest). The Q should be calculated for each spore and the range of Q should be given, and also the average Q.
The last picture in this observation is done pretty well:
http://mushroomobserver.org/64805

The numbers in parenthesis are the largest and smallest values observed. N is the number of spores measured. Me is the mean spore size and Qe is the mean Q value.
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Hags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 6 months
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Ya I had the area algorithm already handy. It will take a couple days to put together the Q, but damn it you've got me set on getting some real data out of this. As said, I do histopath data analysis for a living, and been published; with some legit results this is completely viable for a publication. will post soon, thanks AR
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Wallpapereater
Hoppin Jalapeño



Registered: 02/13/11
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Re: Cambodian 40x Microscopy [Re: Hags]
#14264137 - 04/09/11 07:53 PM (12 years, 10 months ago) |
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Really cool stuff man!
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Tritium



Registered: 03/22/11
Posts: 152
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so as long as your work is finished you can use some of the lab equipment for non-work related things?
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ic3.qu33n
Muff Muncher


Registered: 03/24/11
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Re: Cambodian 40x Microscopy [Re: Tritium]
#14316209 - 04/19/11 09:13 AM (12 years, 10 months ago) |
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thats sooo neat looking! i wanna be able to observe on that level someday. Start small and build I guess.... I got a magnifying glass for now to view my rhizo growth...
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Disclaimer: Anything, I've said I do or done, or say is a complete facade of my dog Dr. Myco McRufferton's dream lifestyle. Freestone Texas Grow << Freestone Texas and B+ Prints available for Trade >> PM me My 5g Bucket Dehydrator Tek
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Mr.E
Stranger
Registered: 03/26/11
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Very interesting Hags, thanks for posting and I hope you can continue your analysis. Your Cambodians seem to be on the small end of wiki claims for Psilocybe cubensis. "Microscopic features: Spores 11.5–17 x 8–11 µm". Not meant to disagree just found it interesting and relevant.
Wiki also claims "The diameter of human hair varies from 17 to 180 µm". I assume this would mean any visible Psilocybe 'spores' are actually clumps?
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fngbronco
Monkey Man



Registered: 09/26/10
Posts: 2,877
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Re: Cambodian 40x Microscopy [Re: Mr.E]
#14347093 - 04/25/11 01:18 AM (12 years, 10 months ago) |
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One of the central spores in that first pic looks like it's growing out. That'd be awesome to put some agar between 2 slides and grow 2 spores out then transfer and get you an isolate!
-------------------- I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need. Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not. -fngbronco Pill Divider Agar Tek
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MolecularConcept
Old School Back Again



Registered: 05/24/10
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Re: Cambodian 40x Microscopy [Re: fngbronco]
#14347246 - 04/25/11 02:10 AM (12 years, 10 months ago) |
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This was a cool read!
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singult



Registered: 05/28/09
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Hags, much thanks for these! I've just started microscopy and what you're laying down is what I'm picking up, with gusto.
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